National Cerebral and Cardiovascular


Research Institute

Department of Research Promotion and Management



Specially Appointed DirectorMasamichi YAMAMOTO
Laboratory ChiefShuji SHIMIZU (Research Promotion)
Yoshitaka FUJIHARA (Animal Resources)
Koji UEMURA (Biomedical Sciences and Information)
Staff ScientistTakuya NISHIKAWA (Research Promotion)
Research FellowIsao KOYAMA (CDC/CRMLN)
Yuichiro ONISHI
Takahito AYANO

Research Promotion and Research Integrity

The animal experiment labs have animal holding areas and advanced facilities intended for large experimental animals to develop clinical research. We believe favorable rearing environments for animals contributes to experimental results with high reliability and reproducibility. We pay close attention to preventing the spread of infectious diseases.

Research Project
Our research group aims to understand and develop therapies for various diseases by measuring and controlling energy metabolism.
ATP, which is produced in energy metabolism, forms the basis of homeostasis by regulating ion concentration, signal transduction, and transcription inside and outside the cell. We have succeeded in developing a technology to visualize ATP dynamics in live mice and rats in vivo for the first time in the world. Using this technology, we are promoting physiological, disease, and pharmacological research focusing on the cardiovascular system, skeletal muscle, and central nerves systems.
In the cardiovascular system, we are working on new measurements of cardiac diseases, energy efficiency and improvement by drugs, and prediction of cardiotoxicity, based on the analysis of ATP dynamics during the beating of the heart and cardiomyocyte. We are also working on the development of new physicochemical therapies based on the analysis of ATP dynamics during the progression of the kidney from acute kidney injury to chronic kidney disease.


  1. Nakajima, N, Ohnishi Y, Yamamoto M, Setoyama D, Imai H, Takenaka T, Matsumoto M, Hosomi K, Saitoh Y, Furue H, Kishima H. Excess intracellular ATP at the lesion site causes at-level neuropathic pain following spinal cord injury. Cellular and Molecular Life Sciences. 2022; 79: 483. doi: 10.1007/s00018-022-04510-z
  2. Kobayashi K, Iwai M, Ono Y, Sun W, Sugimachi M, Kusano K, Shishido T. Magnetocardiography Current source estimation using multiple spatial filters. J Magn Soc Jp. 2021; 45: 131-135. doi: 10.3379/msjmag.2109R003.
  3. Nishikawa K, Seno S, Yoshihara T, Narazaki A, Sugiura Y, Shimizu R, Kikuta J, Sakaguchi R, Suzuki N, Takeda N, Semba H, Yamamoto M, Okuzaki D, Motoka D, Kobayashi Y, Suematsu M, Koseki H, Matsuda H, Yamamoto M, Tobita S, Mori Y, Ishii M. Osteoclasts adapt to physioxia perturbation through DNA demethylation. EMBO Reports. 2021; 18: e53035. doi: 10.15252/embr.202153035.
  4. He J, Yamamoto M, Sumiyama K, Konagaya Y, Terai K, Matsuda M, Sato S. Two-photon AMPK and ATP imaging reveals the bias between rods and cones in glycolysis utility. FASEB J. 2021; 35: e21880. doi: 10.1096/fj.202101121R.
  5. Ohnishi Y, Yamamoto M, Sugiura Y, Setoyama D, Kishima H. Rostro-caudal different energy metabolism leading to differences in degeneration in spinal cord injury. Brain Communications. 2021; 3: fcab058. doi: 10.1093/braincomms/fcab058.
  6. Iwakami N, Nagai T, Furukawa TA, Tajika A, Onishi A, Nishimura K, Ogata S, Nakai M, Takegami M, Nakano H, Kawasaki Y, Alba AC, Guyatt GH, Shiraishi Y, Kohsaka S, Kohno T, Goda A, Mizuno A, Yoshikawa T, Anzai T. Optimal sampling in derivation studies was associated with improved discrimination in external validation for heart failure prognostic models. J Clin Epidemiol. 2020; 121: 71-80. doi: 10.1016/j.jclinepi.2020.01.011.

last updated : 2024/04/25

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