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Inactivation of Nevus Tissue by High Hydrostatic Pressure Method

The nevus tissue is known as the skin teratoma, and congenital giant pigmented nevus is one of the nevus tissue. This tissue contains nevus cells into the epidermis, and melanin pigment is deposited in the tissue. Therefore, the nevus tissue has a cancer risk at a rate of several percent. In general, the nevus tissue is extirpated and replaced with the autologous skin graft which is prepared by the expander in patient body during few months. The patients have to receive several time operations before the replacement. The procedure is invasive manner with costly and time consuming for the patient.

To solve this problem, we develop the new transplantation procedure for giant nevus skin by using high pressure method. We named this procedure "tissue-recycling transplantation". This research is joint project with the Kansai Medical University. In the previous experiments, we revealed that the nevus cells in the tissue could be inactivated by pressure treatment at 200 MPa during 10 min (Figure). The animal experimental results indicated that the inactivated tissue could adhere on the skin defect. Therefore, the extirpation and transplantation are achieved during one operation with less burden on a patient. This clinical study is underway in the Kansai Medical University. We hope that many patients are freed from the pain and skin cancer problem by tissue-recycle transplantation.

Figure High hydrostatic pressure treatment for nevus skin tissue


  1. Morimoto N, Mahara A, Shima K, Ogawa M, Jinno C, Kakudo N, Kusumoto K, Fujisato T, Suzuki S, Yamaoka T.The Rapid Inactivation of Porcine Skin by Applying High Hydrostatic Pressure without Damaging the Extracellular Matrix. Biomed Res Int. 587247 (2015).
  2. Liem PH, Morimoto N, Mahara A, Jinno C, Shima K, Ogino S, Sakamoto M, Kakudo N, Inoie M, Kusumoto K, Fujisato T, Suzuki S, Yamaoka T., Preparation of Inactivated Human Skin Using High Hydrostatic Pressurization for Full-Thickness Skin Reconstruction., PLoS One. 10(7), e0133979 (2015).
  3. Chizuru Jinno, Naoki Morimoto, Atsushi Mahara, Liem PH, Sakamoto M, Ogino S, Kakudo N, Inoie M, Fujisato T, Kusumoto K, Suzuki S, Yamaoka T., Inactivation of human nevus tissue using high hydrostatic pressure for autologous skin reconstruction: a novel treatment for giant congenital melanocytic nevi. Tissue Eng., Tissue Eng Part C Methods. 2015.
  4. Mahara A, Sago M, Yamaguchi H, Ehashi T, Minatoya K, Tanaka H, Nakatani T, Moritan T, Fujisato T, Yamaoka T. Micro-CT evaluation of high pressure-decellularized cardiovascular tissues transplanted in rat subcutaneous accelerated-calcification model. J Artif Organs. 18, 143-150 (2015).
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