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MRI tracking of living stem cells

In recent years, tissue regeneration by transplanting mesenchymal stem cells (MSCs) and various functional cells derived from induced pluripotent stem (iPS) cells is attracting great attentions. However, it is difficult to clarify the engraftment ratio, migration, and survival time in vivo. We have been focusing on the magnetic resonance imaging (MRI) for cell monitoring because of its better advantages in observation depth and resolution compared with optical imaging system. We synthesized water-soluble contrast agents by introducing gadolinium (Gd) chelate (DOTA) to the dextran or PVA and labeled MSCs, iPScells, and other cells with them by electroporation. The fate of these cells was successfully clarified by MRI.

Fig.1 Polymeric MRI contrast agent for transplanted stem cell tracking

Publications

  1. Y. Tachibana, J. Enmi, C. A. Agudelo, H. Iida, and T. Yamaoka, Long-term/bioinert labeling of rat mesenchymal stem cells with PVA-Gd conjugates and MRI monitoring of the labeled cell survival after intramuscular transplantation, Bioconjugate Chemistry, 25(7), 1243-51 (2014)
  2. C. A. Agudelo and *T. Yamaoka, Magnetic resonance imaging detection of an uncommon granuloma formation after endothelial progenitor cells transplantation, Bio-Medical Materials and Engineering, 23, 555-566 (2013)C. A. Agudelo, Y. Tachibana, A. F. Huatado, T. Ose, H. Iida, and *T. Yamaoka, The use of magnetic resonance cell tracking to monitor endothelial progenitor cells in a rat hindlimb ischemic model, Biomaterials, 33, 2439-2448 (2012)
  3. C. A. Agudelo, Y. Tachibana, and *T. Yamaoka, Synthesis, properties, and endothelial progenitor cells labeling stability of dextranes as polymeric MRI contrast agents, Journal of Biomaterials Applications, 28(3) 473-480 (2012)
  4. C. A. Agudelo, Y. Tachibana, N. Teramoto, H. Iida, and *T. Yamaoka, Long term in vivo MRI tracking of endothelial progenitor cells transplanted in rat ischemic limbs and their angiogenic potential, Tissue Engineering Part A., 17(15/16), 2079-2089 (2011)
  5. Y. Tachibana, J. Enmi, A.Mahara, H. Iida, and *T. Yamaoka, Design and characterization of a polymeric MRI contrast agent based on PVA for in vivo living-cell tracking, Contrast Media and Molecular Imaging, 5, 309-317(2010)
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