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Acellular Small Diameter Long Bypass Grafts

Researchers have attempted to develop efficient antithrombogenic surfaces, and yet small-caliber artificial vascular grafts are still unavailable. Here, we demonstrate the excellent patency of tissueengineered small-caliber long-bypass grafts measuring 20e30 cm in length and having a 2-mm inner diameter. The inner surface of an acellular ostrich carotid artery was modified with a novel heterobifunctional peptide composed of a collagen-binding region and the integrin a4b1 ligand, REDV. Six grafts were transplanted in the femoralefemoral artery crossover bypass method. Animals were observed for 20 days and received no anticoagulant medication. No thrombogenesis was observed on the luminal surface and five cases were patent. In contrast, all unmodified grafts became occluded, and severe thrombosis was observed. The vascular grafts reported here are the first successful demonstrations of short-term patency at clinically applicable sizes.

Fig.1 Prepared acellular blood vessel and its transplantation

Publications

  1. A. Mahara, S. Somekawa, N. Kobayashi, Y. Hirano, Y. Kimura, T. Fujisato, and *T. Yamaoka, Tissue-Engineered Acellular Small Diameter Long-bypass Grafts with neointima-inducing activity, Biomaterials, 58, 54-62 (2015)
  2. *S. Sakakibara, Y. Ishida, K. Hashikawa, T. Yamaoka, and H. Terashi, Intima/medulla reconstruction and vascular contraction-relaxation recovery for acellular small diameter vessels prepared by hyperosmotic electrolyte solution treatment, Journal of Artificial Organs, 17, 169-177 (2014)
  3. T. Ehashi, A. Nishigaito, T. Fujisato, Y. Moritan, and *T. Yamaoka, Peripheral nerve regeneration and electrophysiological recovery with CIP-treated allogeneic acellular nerve, Journal of Biomaterials Science Polymer Edition, 22, 627-640 (2011)
  4. *K. Sawada, D. Terada, T. Yamaoka, S. Kitamura, and T. Fujisato, Cell removal with supercritical carbon dioxide for acellular artificial tissue, Journal of Chemical Technology & Biotechnology, 83(6), 943-949 (2008)
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